Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mitochondria01:37

Mitochondria

Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
Role of Hippocampus in Memory01:19

Role of Hippocampus in Memory

The hippocampus, a critical brain structure, plays an essential role in memory processing, particularly in the formation and retrieval of memory. This small, seahorse-shaped region is located within the medial temporal lobe, with one hippocampus in each brain hemisphere. Experimental studies involving lesions in the hippocampi of rats have demonstrated significant impairments in tasks such as object recognition and maze navigation, indicating the hippocampus involvement in both recognition and...
Dementia l: Introduction01:22

Dementia l: Introduction

Dementia is an acquired, progressive syndrome characterized by a decline in multiple cognitive domains severe enough to impair daily functioning and reduce independence. Although memory loss is a central feature, the diagnosis requires additional deficits involving language, executive function, visuospatial skills, judgment, calculation, or abstract reasoning. These cognitive impairments reflect underlying neurodegenerative or vascular processes that gradually disrupt neuronal networks...
Alzheimer Disease l: Introduction01:29

Alzheimer Disease l: Introduction

Alzheimer disease is a chronic, progressive, and irreversible neurodegenerative disorder and the most common cause of dementia in older adults. It leads to gradual neuronal loss, causing cognitive decline, behavioral changes, and loss of functional independence.Risk Factors and EtiologyThe disease is multifactorial. Age is the strongest risk factor, with prevalence doubling every 5 years after age 65. Genetic factors include mutations in genes such as APP, PSEN1, and PSEN2, which are associated...
Aging01:26

Aging

Aging is a complex biological phenomenon influenced by various processes that affect cellular and systemic functions. Several prominent theories attempt to explain its mechanisms, highlighting cellular limitations, oxidative damage, and hormonal changes as central factors in aging.
Cellular Clock Theory
The cellular clock theory posits that the human lifespan is closely tied to the finite capacity of cells to divide, a phenomenon governed by telomeres, which are protective caps at the ends of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

First molecular detection of <i>Theileria haneyi</i> infection in horses in Southern Spain.

Frontiers in veterinary science·2026
Same author

Neuroprotective Role of Cannabinoid CB<sub>1</sub> and GPR55 Receptors in a Cell Model of Multiple Sclerosis.

Molecular neurobiology·2026
Same author

Nationwide Implementation of Double Reflex Testing for Hepatitis Delta in Spain: Results From the Retrospective Phase of the Spain-DDR Study.

Alimentary pharmacology & therapeutics·2026
Same author

Discrete interneuron subsets participate in GluN1/GluN3A excitatory glycine receptor (eGlyR)-mediated regulation of hippocampal network activity throughout development and evolution.

Research square·2025
Same author

A case series of adenomyoepithelioma of the breast with a case of malignant adenomyoepithelioma misinterpreted as polymorphous adenocarcinoma.

AME case reports·2025
Same author

Apolipoprotein D Expression Dynamics During Cuprizone-Induced Demyelination and Remyelination in a Mouse Model of Multiple Sclerosis.

International journal of molecular sciences·2025

Related Experiment Video

Updated: Jul 9, 2026

Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology
14:57

Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology

Published on: March 23, 2011

Hippocampal mitochondrial dysfunction in rat aging.

Ana Navarro1, José M López-Cepero, Manuel J Bández

  • 1Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Plaza Fragela 9, 11003-Cádiz, Spain. ana.navarro@uca.es

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
|December 14, 2007
PubMed
Summary
This summary is machine-generated.

Aging impairs hippocampus mitochondria function, reducing energy supply and increasing oxidative damage. This mitochondrial dysfunction is linked to age-related cognitive decline in rats.

More Related Videos

Using Live Cell STED Imaging to Visualize Mitochondrial Inner Membrane Ultrastructure in Neuronal Cell Models
08:48

Using Live Cell STED Imaging to Visualize Mitochondrial Inner Membrane Ultrastructure in Neuronal Cell Models

Published on: June 30, 2023

Related Experiment Videos

Last Updated: Jul 9, 2026

Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology
14:57

Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology

Published on: March 23, 2011

Using Live Cell STED Imaging to Visualize Mitochondrial Inner Membrane Ultrastructure in Neuronal Cell Models
08:48

Using Live Cell STED Imaging to Visualize Mitochondrial Inner Membrane Ultrastructure in Neuronal Cell Models

Published on: June 30, 2023

Area of Science:

  • Neuroscience
  • Gerontology
  • Mitochondrial Biology

Background:

  • Aging is associated with progressive decline in brain function.
  • Mitochondrial dysfunction is implicated in age-related neurological disorders.

Purpose of the Study:

  • To investigate the effects of aging on hippocampus mitochondrial function and oxidative stress in rats.
  • To correlate mitochondrial dysfunction with enzymatic activity and oxidative damage in the aging hippocampus.

Main Methods:

  • Assessed mitochondrial respiration and enzymatic activities (Complex I, IV, nitric oxide synthase) in rat hippocampus at different ages (4, 12, 20 months).
  • Quantified oxidative damage using thiobarbituric-acid reactive substances (TBARS) and protein carbonyls.
  • Utilized histochemical assays for mitochondrial complex IV staining.

Main Results:

  • Aged and senescent rats exhibited significant decreases in state 3 respiration and activities of mitochondrial complexes I and IV in the hippocampus.
  • Mitochondrial nitric oxide synthase activity was also reduced with age.
  • Increased oxidative damage (TBARS, protein carbonyls) was observed in the hippocampus of aged rats, correlating with reduced enzymatic activity.

Conclusions:

  • Aging leads to hippocampus mitochondrial dysfunction characterized by impaired electron transfer and increased oxidative stress.
  • This dysfunction results in reduced energy supply, contributing to age-related hippocampus dysfunction.
  • Mitochondrial health is crucial for maintaining cognitive function during aging.