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Related Experiment Video

Updated: Jul 9, 2026

Functional Analysis of the Larval Feeding Circuit in Drosophila
09:23

Functional Analysis of the Larval Feeding Circuit in Drosophila

Published on: November 19, 2013

Drosophila eggshell is patterned by sequential action of feedforward and feedback loops.

Nir Yakoby1, Jessica Lembong, Trudi Schüpbach

  • 1Department of Chemical Engineering and Lewis-Sigler Institute for Integrative Genomics, Princeton University, NJ 08544, USA.

Development (Cambridge, England)
|December 14, 2007
PubMed
Summary

Drosophila oogenesis involves EGFR and Dpp pathways regulating Broad (Br) gene expression. A feedback loop involving Br and its target thickveins (tkv) refines dorsal eggshell patterning.

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Area of Science:

  • Developmental Biology
  • Molecular Genetics
  • Cell Signaling

Background:

  • Drosophila oogenesis establishes dorsal eggshell structures via EGFR and Dpp pathways.
  • Broad (Br) is a key transcription factor in dorsal follicle cell patterning, regulated by EGFR and Dpp.
  • Understanding the interplay between these pathways is crucial for deciphering developmental gene regulation.

Purpose of the Study:

  • To investigate the regulatory mechanisms controlling Broad (Br) expression during Drosophila oogenesis.
  • To elucidate the role of Br in the feedback regulation of Dpp signaling.
  • To model the dynamics of Br expression driven by feedforward and feedback loops.

Main Methods:

  • Analysis of gene expression patterns in Drosophila follicle cells.

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Upright Imaging of Drosophila Egg Chambers
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Imaging Calcium in Drosophila at Egg Activation

Published on: August 6, 2016

Related Experiment Videos

Last Updated: Jul 9, 2026

Functional Analysis of the Larval Feeding Circuit in Drosophila
09:23

Functional Analysis of the Larval Feeding Circuit in Drosophila

Published on: November 19, 2013

Upright Imaging of Drosophila Egg Chambers
12:29

Upright Imaging of Drosophila Egg Chambers

Published on: March 13, 2015

Imaging Calcium in Drosophila at Egg Activation
07:45

Imaging Calcium in Drosophila at Egg Activation

Published on: August 6, 2016

  • Investigating the regulation of Broad (Br) and thickveins (tkv) by EGFR and Dpp signaling.
  • Developing a computational model for gene regulatory network dynamics.

    • Main Results:

    • EGFR signaling induces Br, while Dpp signaling overrides this induction in specific cell populations.
    • Changes in Br expression pattern affect Dpp receptor thickveins (tkv) expression.
    • Br acts in a negative feedback loop to control Dpp signaling and its own repression.

    Conclusions:

    • A model of sequential feedforward and feedback loops explains Br expression dynamics.
    • The feedforward loop establishes spatial patterns, while the feedback loop provides temporal modulation.
    • This mechanism illustrates how complex gene expression patterns arise from simple regulatory network motifs.