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Updated: Jul 9, 2026
Germ Cell Transplantation and Testis Tissue Xenografting in Mice
Published on: February 6, 2012
[Cloning of gdnf in the mouse testis and its expression in sertoli cells]
Cui-mi Duan1, En-zhong Li, Shi-qing Zhang
1Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China. duancm2006@yahoo.com.cn
Objective:
To clone the glial cell line-derived neurotrophic factor (GDNF) from the mouse testis, construct the eukaryotic expression vector and transfect this vector into Sertoli cells in order to use the gdnf-transfected Sertoli cells as the feeder layer to cultivate spermatogonial stem cells (SSCs).
Methods:
Total RNA was extracted from the testes of normal mature mice and gdnf was cloned and amplified using RT-PCR, inserted into the eukaryotic expression vector and transfected into sertoli cells (TM4 cell line). Immunofluorescence with anti-GDNF antibodies was performed at 40 h following the transfection.
Results:
gdnf cDNA was cloned successfully, and GDNF expressed after transfected into Sertoli cells.
Conclusion:
This study provides a basis for culturing SSCs with gdnf-transfected Sertoli cells as the feeder layer.
