Related Experiment Video
Updated: Jul 9, 2026
Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
Published on: July 6, 2016
Highly effective DNA photocleavage by novel "rigid" Ru(bpy)(3)-4-nitro- and -4-amino-1,8-naphthalimide conjugates
Gary J Ryan1, Susan Quinn, Thorfinnur Gunnlaugsson
1School of Chemistry, Centre for Synthesis and Chemical Biology (CSCB), University of Dublin, Trinity College Dublin, Dublin 2, Ireland.
Abstract:
The synthesis of the two novel 1,8-naphthalimideruthenium conjugates Ru-Nap-NO(2) and Ru-Nap-NH(2) and their photophysical evaluation upon interaction with DNA is reported. Significant changes were seen in both the absorption and emission spectra upon interaction of both conjugates with DNA, from which large binding constants were determined. Moreover, highly efficient DNA cleavage was observed upon irradiation for 5 min, during which supercoiled DNA was converted to nicked and linear DNA by Ru-Nap-NH(2).
