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Updated: Jul 9, 2026

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
Published on: February 16, 2014
Direct selection and phage display of a Gram-positive secretome
Dragana Jankovic1, Michael A Collett, Mark W Lubbers
1Institute of Molecular Biosciences, Massey University, Palmerston North, New Zealand. d.jankovic@massey.ac.nz
Abstract:
Surface, secreted and transmembrane protein-encoding open reading frames, collectively the secretome, can be identified in bacterial genome sequences using bioinformatics. However, functional analysis of translated secretomes is possible only if many secretome proteins are expressed and purified individually. We have now developed and applied a phage display system for direct selection, identification, expression and purification of bacterial secretome proteins.
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