Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Riboswitches01:56

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
Transcriptional Regulation: Riboswitches01:23

Transcriptional Regulation: Riboswitches

Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An optimized prime editing system for precise genome editing in soybean.

Plant communications·2026
Same author

Reusing the workhorse: Outcomes after secondary inset of paraspinous muscle flaps.

Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2026
Same author

Role of the anti-Shine-Dalgarno sequence of 16S rRNA in Flavobacterium johnsoniae.

Nucleic acids research·2026
Same author

Comparative efficacy of acute targeted muscle reinnervation after upper vs. lower limb amputation: A retrospective, global analysis.

Journal of clinical orthopaedics and trauma·2026
Same author

The role of RARG in solid tumor progression and therapeutic potential.

Discover oncology·2026
Same author

Epithelial Cell-Specific Prognostic Signature (FTH1, RIT1, WASL, NDRG2, KIFC3) Stratifies Cervical Cancer Patients and Correlates With Immune Infiltration.

Human mutation·2026

Related Experiment Video

Updated: Jul 9, 2026

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
09:05

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Published on: March 11, 2020

Small RNAs in tomato fruit and leaf development.

Asuka Itaya1, Ralf Bundschuh, Anthony J Archual

  • 1Department of Plant Cellular and Molecular Biology, Ohio State University, Columbus, Ohio 43210, USA.

Biochimica Et Biophysica Acta
|December 15, 2007
PubMed
Summary
This summary is machine-generated.

Researchers identified over 1000 small RNAs in tomato, with most being unique to the plant. This discovery aids in understanding how small RNAs regulate gene expression and influence the evolution of fruit and leaf development.

More Related Videos

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
10:08

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Published on: March 5, 2017

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
14:41

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

Published on: July 11, 2020

Related Experiment Videos

Last Updated: Jul 9, 2026

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
09:05

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Published on: March 11, 2020

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
10:08

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Published on: March 5, 2017

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
14:41

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

Published on: July 11, 2020

Area of Science:

  • Plant biology
  • Developmental biology
  • Genomics

Background:

  • Tomato fruit and leaf development are key models for studying organ evolution.
  • Gene regulation plays a crucial role in developmental pathways.

Purpose of the Study:

  • To identify and characterize small RNAs in tomato fruit and leaves.
  • To investigate the role of small RNAs in the evolution of plant organ development.
  • To provide a public resource for tomato small RNA data.

Main Methods:

  • Small RNA sequencing was performed on tomato fruit and leaf tissues.
  • Northern hybridization was used to confirm the expression of selected microRNAs.
  • A searchable website was created to host the identified small RNA sequences and associated data.

Main Results:

  • Over 350 small RNAs were identified in tomato fruit and over 700 in tomato leaves.
  • More than 90% of the identified small RNAs were unique to tomato, excluding conserved microRNAs.
  • Expression of both conserved and novel putative microRNAs was confirmed.

Conclusions:

  • The identified small RNAs provide a foundation for comparative studies on small RNA-mediated gene regulation.
  • These findings contribute to understanding the evolution of distinct developmental pathways for fruits and leaves.
  • A publicly accessible database of tomato small RNAs and their targets is now available.