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Updated: Jul 9, 2026

Cell Co-culture Patterning Using Aqueous Two-phase Systems
Published on: March 26, 2013
Protein partitioning in poly(ethylene glycol)/sodium polyacrylate aqueous two-phase systems
Hans-Olof Johansson1, Flavio Musa Magaldi, Eloi Feitosa
1Department of Biochemical and Pharmaceutical Technology, University of São Paulo, Av. Prof. Lineu Prestes, 580 Bloco 16, Cidade Universitaria, 05508-000 São Paulo, Brazil. Hans-Olof.johansson@biochemistry.lu.se
Abstract:
The partition of hemoglobin, lysozyme and glucose-6-phosphate dehydrogenase (G6PDH) in a novel inexpensive aqueous two-phase system (ATPS) composed by poly(ethylene glycol) (PEG) and sodium polyacrylate (NaPA) has been studied. The effect of NaCl and Na(2)SO(4), pH and PEG molecular size on the partitioning has been studied. At high pH (above 9), hemoglobin partitions strongly to the PEG-phase. Although some precipitation of hemoglobin occurs, high recovery values are obtained particularly for lysozyme and G6PDH. The partitioning forces are dominated by the hydrophobic and electrochemical (salt) effects, since the positively charged lysozyme and negatively charged G6PDH partitions to the non-charged PEG and the strongly negatively charged polyacrylate enriched phase, respectively.
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