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Updated: Jul 9, 2026

Mouse Mammary Epithelial Cells form Mammospheres During Lactogenic Differentiation
Published on: October 6, 2009
A role for microRNAs in maintenance of mouse mammary epithelial progenitor cells
Ingrid Ibarra1, Yaniv Erlich, Senthil K Muthuswamy
1Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.
Abstract:
microRNA (miRNA) expression profiles are often characteristic of specific cell types. The mouse mammary epithelial cell line, Comma-Dbeta, contains a population of self-renewing progenitor cells that can reconstitute the mammary gland. We purified this population and determined its miRNA signature. Several microRNAs, including miR-205 and miR-22, are highly expressed in mammary progenitor cells, while others, including let-7 and miR-93, are depleted. Let-7 sensors can be used to prospectively enrich self-renewing populations, and enforced let-7 expression induces loss of self-renewing cells from mixed cultures.
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