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08:48
Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays
Published on: November 29, 2014
Some natural compounds enhance N epsilon-(carboxymethyl)lysine formation
Yukio Fujiwara1, Naoko Kiyota, Keita Motomura
1Department of Medical Biochemistry, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
Annals of the New York Academy of Sciences
|December 15, 2007
Summary
Advanced glycation end product inhibitors, like pyridoxamine, can prevent diabetic complications. However, some natural compounds, such as quercetin, may paradoxically increase N epsilon-(carboxyethyl)lysine (CML) formation.
Area of Science:
- Biochemistry
- Endocrinology
- Pharmacology
Background:
- Advanced glycation end products (AGEs), particularly N epsilon-(carboxyethyl)lysine (CML), are implicated in diabetic complications.
- Pyridoxamine, an inhibitor of AGE formation, shows protective effects against retinopathy and neuropathy in diabetic rats.
- The effect of natural compounds on CML formation is complex, with some potentially exacerbating the process.
Purpose of the Study:
- To investigate the impact of various natural compounds on the formation of N epsilon-(carboxyethyl)lysine (CML).
- To identify natural compounds that inhibit or enhance CML formation under conditions mimicking the Maillard reaction.
Main Methods:
- Enzyme-linked immunosorbent assay (ELISA) was used to quantify CML formation.
- Bovine serum albumin was incubated with ribose for 7 days to induce CML formation.
- The effects of natural compounds, including desgalactotigonin, quercetin, and acteoside, were assessed.
Main Results:
- Significant CML formation was observed in bovine serum albumin incubated with ribose.
- Desgalactotigonin demonstrated inhibitory effects on CML formation.
- Quercetin and acteoside were found to enhance CML formation.
Conclusions:
- Natural compounds exhibit varied effects on CML formation, with some acting as inhibitors and others as enhancers.
- This highlights the need for careful evaluation of natural compounds intended for managing diabetic complications.
- Further research is warranted to understand the mechanisms behind these differential effects and their clinical implications.
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