Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Strain-Dependent Functional Variation of a Sex Specific Sox9 Enhancer.

Genes to cells : devoted to molecular & cellular mechanisms·2026
Same author

Prognostic outcomes of adjuvant chemotherapy in postoperative patients with cancer undergoing hemodialysis versus non-hemodialysis patients: a retrospective nationwide cohort study.

BMC cancer·2026
Same author

Genomic Profiling of Cancerous Areas, Dysplasia, and Background Mucosa in Ulcerative Colitis-associated Colorectal Cancer.

Anticancer research·2026
Same author

Phenotypically and functionally unique CD86 expression CD8 T cell subset shapes immune regulation in the tumor microenvironment.

Journal of advanced research·2026
Same author

Genomic and Transcriptomic Analysis of High-Grade Endometrial Carcinoma Reveals Biological Heterogeneity and Molecular Classification Challenges.

Cancer research communications·2026
Same author

Study protocol for a phase I investigator-initiated clinical trial of E7820 in Japanese patients with unresectable solid tumours: CIRCUS trial (NCCH2303).

Japanese journal of clinical oncology·2026

Related Experiment Video

Updated: Jul 9, 2026

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays
10:58

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays

Published on: December 3, 2010

Profiling of microRNA expression by mRAP.

Shuji Takada1, Hiroyuki Mano

  • 1Division of Functional Genomics, Jichi Medical University, 3311-1 Yakushiji, Shimotsukeshi, Tochigi 329-0498, Japan.

Nature Protocols
|December 15, 2007
PubMed
Summary

MicroRNA amplification profiling (mRAP) offers sensitive miRNA expression analysis. This method efficiently characterizes miRNA profiles from minimal cell samples within 10 days.

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • MicroRNAs (miRNAs) are crucial regulators of gene expression.
  • Accurate miRNA expression profiling is essential for biological research and diagnostics.
  • Existing methods may require substantial starting material, limiting applications.

Purpose of the Study:

  • To present a sensitive and efficient method for miRNA expression profiling.
  • To enable miRNA analysis from small quantities of biological samples.
  • To establish a protocol for rapid miRNA characterization.

Main Methods:

  • Utilizes microRNA amplification profiling (mRAP), incorporating a 5' adaptor and SMART reaction.
  • Generates miRNA-derived cDNAs flanked by synthesized oligomers.

More Related Videos

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
16:24

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
09:26

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

Published on: January 22, 2014

Related Experiment Videos

Last Updated: Jul 9, 2026

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays
10:58

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays

Published on: December 3, 2010

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
16:24

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
09:26

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

Published on: January 22, 2014

  • Employs PCR amplification, concatemerization, and nucleotide sequencing for analysis.

    • Main Results:

    • mRAP provides sensitive determination of miRNA expression profiles.
    • The method yields millions of miRNA-derived clones from as few as 1 x 10(4) cells.
    • Protocol completion time is 10 days.

    Conclusions:

    • mRAP is a powerful tool for characterizing miRNA expression profiles.
    • The method is suitable for limited starting materials like clinical specimens or embryonic tissues.
    • mRAP significantly advances the ability to study miRNA expression in challenging samples.