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Updated: Jul 9, 2026
Near Infrared Photoimmunotherapy for Mouse Models of Pleural Dissemination
Published on: February 9, 2021
Anti-tumor effect of PDT using Photofrin in a mouse angiosarcoma model
Iizuka Jin1, Morimoto Yuji, Nakagishi Yoshinori
1Department of Dermatology, Japan Self Defense Force Central Hospital, 1-2-24 Ikejiri, Setagaya, Tokyo, 154-8532, Japan. jin_iizuka@mnx.jp
Abstract:
Angiosarcoma, a malignant tumor of vascular endothelial cell origin, is a lethal disease for which complete cure is rarely seen. The objective of this study was to determine the efficiency of photodynamic therapy (PDT) as a new treatment for angiosarcoma. PDT (630 nm, 25 J/cm2) using Photofrin for a mouse angiosarcoma cell line of human origin (ISOS-1) showed that the rate of cell death increased with increase in the concentration of a photosensitizer (LD50: approximately 2 microg/ml). Furthermore, PDT (630 nm, 100 J/cm2) with Photofrin (5 mg/kg, i.v.) in mice transplanted with ISOS-1 cells resulted in complete disappearance of the tumor in 40% of mice and marked inhibition of tumor growth in the remaining 60%. Significant increases in TUNEL-positive cells and Ki-67-positive cells ware seen 4 h after PDT, indicating that PDT led to not only cell death but also inhibition of the proliferation of angiosarcoma cells. The results show that PDT is effective for treatment of angiosarcoma.
Insights
Photodynamic therapy (PDT) effectively treats angiosarcoma, a rare vascular cancer. This study shows PDT significantly reduces tumor size and cell proliferation, offering a promising new treatment option.
Area of Science:
- Oncology
- Biomedical Engineering
- Photomedicine
Background:
- Angiosarcoma is a rare and aggressive vascular endothelial cancer with limited treatment options.
- Complete cure for angiosarcoma is rarely achieved with current therapies.
Purpose of the Study:
- To evaluate the efficacy of photodynamic therapy (PDT) as a novel treatment for angiosarcoma.
- To determine the impact of PDT on angiosarcoma cell death and proliferation.
Main Methods:
- In vitro studies using a human angiosarcoma cell line (ISOS-1) treated with Photofrin-based PDT.
- In vivo studies involving ISOS-1 cell transplantation in mice, followed by Photofrin-based PDT.
- Assessment of cell death via TUNEL staining and proliferation via Ki-67 staining post-PDT.
Main Results:
- PDT demonstrated a dose-dependent increase in angiosarcoma cell death in vitro.
- In vivo, PDT resulted in complete tumor disappearance in 40% of mice and significant tumor growth inhibition in 60%.
- PDT significantly increased TUNEL-positive (apoptotic) and Ki-67-positive (proliferating) cells, indicating both cell death and reduced proliferation.
Conclusions:
- Photodynamic therapy (PDT) is an effective treatment modality for angiosarcoma.
- PDT induces both cell death and inhibits proliferation in angiosarcoma.
- PDT presents a promising therapeutic strategy for angiosarcoma patients.
