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Updated: Jul 9, 2026

Live Cell Imaging of Microtubule Cytoskeleton and Micromechanical Manipulation of the Arabidopsis Shoot Apical Meristem
Published on: May 23, 2020
Cryopreservation of shoot tips and meristems
Erica E Benson1, Keith Harding, Jason W Johnston
1DAMAR, Research Scientists, Conservation, Environmental Science and Biotechnology, Cupar, Fife, Scotland, UK.
Abstract:
Shoot-tip meristem cryopreservation methodologies are reported for the complementary cryoprotective strategies of vitrification and equilibrium freezing using traditional controlled-rate freezing and chemical additive cryoprotection. Pregrowth, pretreatment, and cold acclimation approaches for the improvement of tolerance to liquid nitrogen are also presented. The chapter concludes by reporting an analytical protocol that profiles volatile hydrocarbon stress markers (for ethylene, hydroxyl radicals, and lipid peroxidation products) during cryopreservation. This method uses noninvasive headspace sampling and gas chromatography, and it is widely applicable across cryogenic systems.
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