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Updated: Jul 9, 2026
03:53
Development of Multiplex Real-Time RT-qPCR Assays for the Detection of SARS-CoV-2, Influenza A/B, and MERS-CoV
Published on: November 10, 2023
[Identification and typing for adenovirus by multiplex nest-PCR]
Jie Deng1, Yuan Qian, Lin-Qing Zhao
1Laboratory of Virology, Beijing Municipal Laboratory of Infection and Immunity, Capital Institute of Pediatrics, Beijing 100020, China.
Zhonghua Liu Xing Bing Xue Za Zhi = Zhonghua Liuxingbingxue Zazhi
|December 18, 2007
Summary
A novel multiplex nested PCR method accurately identifies adenovirus types 3, 7, 11, and 21 in clinical samples. This rapid, sensitive, and specific technique improves upon traditional methods for adenovirus typing.
Area of Science:
- Molecular biology
- Virology
- Clinical diagnostics
Context:
- Adenovirus infections pose a significant public health challenge.
- Accurate and rapid identification of adenovirus types is crucial for effective patient management.
- Existing diagnostic methods may lack sensitivity or specificity.
Purpose:
- To develop a rapid, sensitive, and specific method for identifying and typing adenoviruses in clinical specimens.
- To design primers targeting the hexon gene for multiplex nested PCR.
- To validate the method's performance against conventional techniques.
Summary:
- A multiplex nested PCR assay was developed using primers targeting the adenovirus hexon gene.
- The assay successfully identified adenovirus types 3, 7, 11, and 21 with specific PCR product sizes.
- The method demonstrated high sensitivity and specificity, outperforming tissue culture and immunofluorescence assays in detecting adenovirus types.
Impact:
- This multiplex nested PCR offers a significant advancement in adenovirus diagnostics.
- The technique enables faster and more accurate identification of adenovirus types from clinical samples.
- Improved diagnostic capabilities can lead to better clinical outcomes and infection control strategies.
