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Updated: Jul 9, 2026

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Two new alkaloids from Brachystemma calycinum and their inhibitory effects on lymphocyte proliferation
Qing Lu1, Li Zhang, Guo-Rong He
1State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650204, PR China.
Abstract:
Two new alkaloids, brachystemidines F (1) and G (2), were isolated from the roots of Brachystemma calycinum. Their structures were established on the basis of detailed spectroscopic analyses, including extensive NMR and HR-MS techniques. Compound 2, which exhibits an unusual N-hydroxydiazenyl (HO-N=N) moiety, is a potent immunosuppressive agent, as demonstrated by inhibition of mouse T- and B-lymphocyte proliferation, with IC50 values of 6.33 and 5.60 microg/ml, resp.
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