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Related Concept Videos

siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
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A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA
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Published on: December 26, 2015

MicroRNAs as targets for antisense-based therapeutics.

Jan Stenvang1, Sakari Kauppinen

  • 1University of Copenhagen, Wilhelm Johannsen Centre for Functional Genome Research, Department of Cellular and Molecular Medicine, Blegdamsvej 3, DK-2200 Copenhagen, Denmark. stenvang@imbg.ku.dk

Expert Opinion on Biological Therapy
|December 18, 2007
PubMed
Summary

Antisense oligonucleotides can inhibit microRNA (miRNA) function by targeting their base pairing with messenger RNAs. Lessons from related strategies may advance clinical trials for these miRNA-antagonizing oligonucleotides.

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Last Updated: Jul 9, 2026

A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA
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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
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09:53

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Area of Science:

  • Molecular Biology
  • Genetics
  • RNA Biology

Background:

  • MicroRNAs (miRNAs) are endogenous non-coding RNAs regulating gene expression post-transcriptionally.
  • Over 5000 miRNA entries are known, implicating them in cellular processes and diseases.
  • miRNA action relies on Watson-Crick base pairing with target messenger RNAs (mRNAs).

Purpose of the Study:

  • To explore the potential of antisense oligonucleotides for inhibiting miRNA function.
  • To leverage existing knowledge from antisense and small-interfering RNA strategies for miRNA antagonism.

Main Methods:

  • Designing high-affinity nucleotide mimics to target and antagonize miRNAs in vivo.
  • Applying design rules, pharmacokinetic, and safety considerations from established oligonucleotide therapies.

Main Results:

  • Antisense oligonucleotides demonstrate potential for specific miRNA inhibition.
  • In vivo antagonism of miRNAs is achievable using nucleotide mimics.

Conclusions:

  • Antisense oligonucleotides are a promising therapeutic strategy for miRNA-related conditions.
  • Knowledge from antisense and small-interfering RNA approaches can guide clinical development of miRNA-antagonizing oligonucleotides.