Related Experiment Video
Updated: Jul 9, 2026

A RAPID Method for Blood Processing to Increase the Yield of Plasma Peptide Levels in Human Blood
Published on: April 28, 2016
Fragments of obestatin as modulators of feed intake, circulating lipids, and stored fat
ShreeRanga Nagaraj1, Muthukumar S Peddha, Uma V Manjappara
1Department of Protein Chemistry and Technology, Central Food Technological Research Institute, Cheluvamba Mansion, Mysore 570020, Karnataka, India.
Abstract:
Obestatin, shown to reduce feed intake and gain in body weight in rodents, is a very attractive candidate to be used against obesity. In this study, we aimed to investigate the relationship between the primary structure and activity of obestatin. Also of interest to us is a peptide of minimal length that closely mimics obestatin. Towards the same, we synthesized rodent obestatin and three overlapping fragments spanning residues 1-13, 6-18, and 11-23 of obestatin. These peptides subsequent to purification and characterization were tested upon adult male mice for their ability to reduce feed intake and gain in body weight. The N-terminal peptide (residues 1-13) mimicked obestatin the closest. The middle fragment (residues 6-18) significantly reduced epididymal fat without much altering feed intake or body weight.
Related Concept Videos
Regulation of Food Intake
Lipid Digestion
Hormonal Regulation
Glucagon-like Receptor Agonists
GLP-1, when administered in high doses intravenously, triggers insulin secretion, inhibits glucagon release, slows gastric emptying, reduces food intake, and restores normal insulin secretion. However, its rapid inactivation by the...
Fats as Energy Storage Molecules
Cholesterol: Significance and Regulation
Considering cholesterol and...

