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Related Experiment Video

Updated: Jul 9, 2026

Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells

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Published on: April 3, 2019

Synaptic terminals from mice midbrain exhibit functional P2X7 receptor.

P Marín-García¹, J Sánchez-Nogueiro, R Gómez-Villafuertes

¹Departamento de Bioquímica, Facultad de Veterinaria, Universidad Complutense de Madrid, Av. Puerta de Hierro s/n, 28040 Madrid, Spain.

|December 18, 2007

Summary

Researchers confirmed a functional P2X(7) receptor in wild type mice midbrain. A less efficient P2X(7)-like receptor was found in knockout models, impacting calcium signaling.

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Area of Science:

Neuroscience
Molecular Biology
Pharmacology

Background:

The P2X(7) receptor, a key ion channel, has been identified in various neural tissues.
Its presence and function in the midbrain, particularly in synaptic terminals, require further elucidation.

Purpose of the Study:

To investigate the expression and functionality of the P2X(7) receptor in wild type mice midbrain.
To characterize the P2X(7) receptor's pharmacological profile and compare it with a P2X(7) knockout model.

Main Methods:

Quantitative real-time PCR, immunocytochemistry, and Western blot assays were used to detect P2X(7) expression.
Microfluorimetric experiments on isolated synaptic terminals assessed receptor functionality.
Pharmacological profiling involved specific agonists and antagonists, including BzATP, BBG, and KN-62.

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Proteomics to Identify Proteins Interacting with P2X2 Ligand-Gated Cation Channels

Proteomics to Identify Proteins Interacting with P2X2 Ligand-Gated Cation Channels

Published on: May 18, 2009

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Last Updated: Jul 9, 2026

Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells

Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells

Published on: April 3, 2019

Proteomics to Identify Proteins Interacting with P2X2 Ligand-Gated Cation Channels

Proteomics to Identify Proteins Interacting with P2X2 Ligand-Gated Cation Channels

Published on: May 18, 2009

Main Results:

Functional P2X(7) receptors were confirmed in wild type mice midbrain, with specific EC(50) and IC(50) values for agonists and antagonists.
A "P2X(7)-like" protein was detected in P2X(7) knockout mice, exhibiting altered pharmacological properties.
Calcium mobilization was significantly higher in wild type mice compared to knockout models.

Conclusions:

The study provides robust evidence for a functional P2X(7) receptor in the wild type mice midbrain.
A distinct, less efficient "P2X(7)-like" receptor exists in the P2X(7) knockout model, affecting cellular responses.