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Updated: Jul 9, 2026

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Large-Scale Screens of Metagenomic Libraries
Published on: May 28, 2007
Rapid DNA library construction for functional genomic and metagenomic screening
Jonathan E Schmitz1, Anu Daniel, Mattias Collin
1Laboratory of Bacterial Pathogenesis and Immunology, Rockefeller University, 1230 York Avenue, Box 172, New York, NY 10021, USA.
Applied and Environmental Microbiology
|December 18, 2007
Abstract:
A rapid protocol was developed for constructing plasmid libraries from small quantities of genomic/metagenomic DNA. The technique utilizes linker amplification with topoisomerase cloning and allows for inducible transcription in Escherichia coli. As proof of principle, several anti-Bacillus lysins were cloned from bacteriophage genomes and an aerolysin was cloned from a metagenomic sample.

