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Using dynamic low-coherence interferometry to image Brownian motion within highly scattering media.

D A Boas, K K Bizheva, A M Siegel

|December 18, 2007

Summary

Dynamic low-coherence interferometry accurately measures Brownian motion in highly scattering media. This technique enables particle characterization in concentrated suspensions, revealing spatial diffusion variations.

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Area of Science:

Biophysics
Optical Physics
Materials Science

Background:

Measuring Brownian motion in highly scattering media is challenging due to light multiple scattering.
Traditional particle characterization methods are limited to optically dilute suspensions.
Understanding particle dynamics in concentrated suspensions is crucial for various applications.

Purpose of the Study:

To develop and validate a method for measuring Brownian motion in highly scattering media.
To apply particle characterization techniques to optically concentrated suspensions.
To image spatial variations of the Brownian diffusion coefficient.

Main Methods:

Dynamic low-coherence interferometry combined with a confocal microscope.
Utilized a coherence gate for strong rejection of multiply scattered light.
Measured Brownian diffusion coefficient and power spectrum broadening.

Main Results:

Successfully measured Brownian motion of submicrometer particles in highly scattering media.
Determined the Brownian diffusion coefficient with accuracy better than 5%.
Imaged spatial variations in the Brownian diffusion coefficient with ~30 mum axial resolution.
Observed power spectrum broadening with increasing sample depth.

Conclusions:

Dynamic low-coherence interferometry is effective for studying particle dynamics in concentrated suspensions.
The technique allows particle characterization previously limited to dilute systems.
Spatial mapping of diffusion coefficients provides new insights into complex media.