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Updated: Jul 9, 2026

Neutron Crystallography Data Collection and Processing for Modelling Hydrogen Atoms in Protein Structures
Published on: December 1, 2020
HBNG: Graph theory based visualization of hydrogen bond networks in protein structures
Abhishek Tiwari1, Vivek Tiwari
1GVK Biosciences, Informatics Division, Hyderabad 500037, India. abhishek.twr@gmail.com
Unlabelled:
HBNG is a graph theory based tool for visualization of hydrogen bond network in 2D. Digraphs generated by HBNG facilitate visualization of cooperativity and anticooperativity chains and rings in protein structures. HBNG takes hydrogen bonds list files (output from HBAT, HBEXPLORE, HBPLUS and STRIDE) as input and generates a DOT language script and constructs digraphs using freeware AT and T Graphviz tool. HBNG is useful in the enumeration of favorable topologies of hydrogen bond networks in protein structures and determining the effect of cooperativity and anticooperativity on protein stability and folding. HBNG can be applied to protein structure comparison and in the identification of secondary structural regions in protein structures.
Availability:
Program is available from the authors for non-commercial purposes.
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