Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Skeletal ("mesenchymal") stem cells for tissue engineering.

Pamela Gehron Robey1, Sergei A Kuznetsov, Mara Riminucci

  • 1Department of Health and Human Services, Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA.

Methods in Molecular Medicine
|December 19, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Improving cost-efficiency in port-site fascial closure: a novel Veress-needle technique and a comprehensive literature review.

Updates in surgery·2026
Same author

3D bone printing via ultrasound-mediated osteogenic differentiation of stem cells (3DBonUS).

Biofabrication·2026
Same author

Lineage topology, replication kinetics and cell cycle synchronization reveal regulated growth dynamics in human bone marrow stromal cell colonies.

Scientific reports·2026
Same author

Disruption of thrombospondin 1/2-integrin β1 axis impairs cell adhesion and tumor growth in intrahepatic cholangiocarcinoma.

Cell death discovery·2026
Same author

Inhibition of endolysosomal two-pore channel 2 (TPC2) induces osteoblast differentiation and matrix mineralization while targeting autophagy.

Journal of endocrinological investigation·2026
Same author

Exploring bone formation mechanism and pattern during RANKL inhibition in a fibrous dysplasia mouse model.

Communications biology·2026
Same journal

Erratum to: Immunotherapeutic Approach to Cancer with Cutaneous DNA Vaccination.

Methods in molecular medicine·2015
Same journal

Methods for cancer gene therapy using tumor suppressor genes.

Methods in molecular medicine·2014
Same journal

Suppression of the human carcinoma phenotype by an antioncogene ribozyme.

Methods in molecular medicine·2014
Same journal

Methods for the use of stromal cells for therapeutic gene therapy.

Methods in molecular medicine·2014
Same journal

Methods for adenovirus-mediated gene transfer to synovium in vivo.

Methods in molecular medicine·2014
Same journal

Methods for gene transfer to synovium.

Methods in molecular medicine·2014
See all related articles

Skeletal stem cells (SSCs) from bone marrow can form bone tissue. These cells show potential for preclinical bone repair models.

Area of Science:

  • Cell Biology
  • Regenerative Medicine
  • Orthopedics

Background:

  • Skeletal stem cells (SSCs), also known as mesenchymal stem cells, reside within bone marrow stromal cells (BMSCs).
  • SSCs possess the capacity for self-renewal and differentiation into multiple skeletal lineages.

Purpose of the Study:

  • To investigate the multipotency and bone-forming capabilities of SSCs.
  • To explore the utility of SSCs in preclinical models for bone repair.

Main Methods:

  • Isolation and culture of SSCs from bone marrow.
  • In vivo transplantation assays to assess differentiation and bone formation.
  • Evaluation of SSC-derived cell strains for bone regeneration potential.

Main Results:

Related Experiment Videos

  • SSCs were successfully isolated as adherent, clonogenic cells in culture.
  • In vivo assays demonstrated the multipotency of SSCs, confirming their ability to differentiate into skeletal tissues.
  • SSCs exhibited competence in forming significant amounts of bone in vivo.

Conclusions:

  • SSCs are multipotent cells capable of differentiating into various skeletal lineages.
  • The bone-forming capacity of SSCs supports their use in developing preclinical models for bone repair strategies.