Related Experiment Video
Updated: Jul 9, 2026
05:49
Protein Engineering by Yeast Surface Display
Published on: November 29, 2024
Fishing for lectins from diverse sequence libraries by yeast surface display - an exploratory study
Stefan Ryckaert1, Nico Callewaert, Pieter P Jacobs
1Department for Molecular Biomedical Research, Unit for Fundamental and Applied Molecular Biology, VIB, Ghent University, Ghent, Belgium.
Glycobiology
|December 19, 2007
Summary
Yeast surface display (YSD) enables cloning of carbohydrate-binding proteins (lectins) from cDNA libraries. This technology facilitates the study of protein-carbohydrate interactions and isolation of specific lectins.
Area of Science:
- Glycomics
- Molecular Biology
- Biotechnology
Background:
- Cloning carbohydrate-binding proteins is crucial for glycomics research.
- Existing methods for lectin discovery present challenges.
- A robust technology platform is needed for expression cloning.
Purpose of the Study:
- To explore yeast surface display (YSD) for interaction-based lectin cloning.
- To evaluate YSD for displaying lectins on yeast surfaces.
- To enable detailed studies of protein-carbohydrate interactions.
Main Methods:
- Utilized yeast surface display (YSD) with Saccharomyces cerevisiae and Pichia pastoris.
- Displayed lectins (galectins, siglecs, C-type lectins) as agglutinin fusions.
- Employed fluorescence-activated cell sorting (FACS) with labeled glycoconjugates for isolation.
Main Results:
- Successfully displayed five lectins from different subfamilies on yeast surfaces.
- Detected carbohydrate-binding activity for galectin-1, galectin-3, and siaoadhesin.
- Isolated proteins with carbohydrate affinity from complex cDNA libraries using FACS.
Conclusions:
- Yeast surface display is a viable technology for lectin cloning and characterization.
- This method facilitates the study of protein-carbohydrate interactions.
- YSD combined with FACS enables specific isolation of carbohydrate-binding proteins.
