Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Three-dimensional microscopy with phase-shifting digital holography.

T Zhang1, I Yamaguchi

  • 1Graduate Course of Science and Engineering, Saitama University, Urawa, Saitama 338-8570, Japan.

Optics Letters
|December 19, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Study of the electron-doping mechanism in single-walled carbon nanotubes using dimethylbenzimidazole.

Faraday discussions·2023
Same author

Pharbitis class-1 knotted-like homeobox gene, PKn3, shares similar characteristics to those of class-2 knotted-like genes.

Plant cell reports·2019
Same author

Can radiological technologists serve as primary screeners of low-dose computed tomography for the diagnosis of lung cancer?

Journal of X-ray science and technology·2018
Same author

Semi Quantification of Gibberellins in the Anthers of Thermosensitive Genetic Male Sterile Rice (Oryza sativa L. cv. PL12).

Bioscience, biotechnology, and biochemistry·2016
Same author

cDNA Subtractive Cloning of Genes Expressed during Early Stage of Appressorium Formation by Magnaporthe grisea.

Bioscience, biotechnology, and biochemistry·2016
Same author

Production of Biodegradable Polyester by a Transgenic Tobacco.

Bioscience, biotechnology, and biochemistry·2016
Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026
Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026
Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026
Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026
Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026
Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026
See all related articles

Phase-shifting digital holography enables 3D microscopy without mechanical parts. This advanced technique reconstructs microscopic object details using light scattering and computer analysis for flexible imaging.

Area of Science:

  • Optics and Photonics
  • Microscopy Techniques
  • Digital Imaging

Background:

  • Traditional microscopy often lacks 3D reconstruction capabilities.
  • Phase-shifting techniques are crucial for accurate optical measurements.
  • Digital holography offers advanced imaging potential.

Purpose of the Study:

  • To develop a phase-shifting digital holography method for microscopy.
  • To reconstruct the complex amplitude of light scattered from 3D microscopic objects.
  • To enable quantitative evaluation and flexible display of microscopic images.

Main Methods:

  • Utilizing phase-shifting digital holography.
  • Recording scattered light from microscopic objects via video camera.
  • Employing computer reconstruction algorithms.

Related Experiment Videos

  • No mechanical movement required during the process.

    • Main Results:

    • Successfully derived the complex amplitude of light.
    • Achieved 3D reconstruction of microscopic objects.
    • Demonstrated flexible display and quantitative evaluation of reconstructed images.
    • Experimental verification with specimens confirmed the method's efficacy.

    Conclusions:

    • Phase-shifting digital holography is a viable and effective microscopy technique.
    • The method offers advantages in flexibility and quantitative analysis over traditional approaches.
    • This technique advances the field of microscopic imaging and analysis.