Direct acoustic profiling of DNA hybridisation using HSV type 1 viral sequences
Yildiz Uludağ1, Xin Li, Heather Coleman
1Akubio Ltd, 181 Cambridge Science Park, Cambridge, UK CB4 0GJ.
The Analyst
|December 19, 2007
Abstract:
We describe the detection of specific, conserved DNA sequences of herpes simplex virus (HSV) type 1 by means of a novel, high sensitivity acoustic biosensor. Repeated assays on planar and polymeric carboxylic acid- and biotin-presenting surface chemistries enabled statistical comparison of assay specificity and sensitivity and evaluation of assay Z-factor scores. Using a three minute hybridisation with NeutrAvidin capture for signal enhancement, it was possible to detect HSV viral nucleic acids at 5.2 x 10(-11) M concentration.
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