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Related Concept Videos

Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...
Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
Genetic Variation01:25

Genetic Variation

Genetic variation is the diversity in DNA sequences found among individuals of the same species. This diversity is crucial for a species' survival because it helps organisms adapt to environmental changes. Genetic variation begins with fertilization, where an egg and sperm cell merge. Each of these cells carries 23 chromosomes, up to 46 in the fertilized egg. Chromosomes are long DNA strands that contain genes, the basic units of heredity.
Genes exist in different versions called alleles, which...
Monohybrid Crosses01:20

Monohybrid Crosses

Overview
Position-effect Variegation02:32

Position-effect Variegation

In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
Dihybrid Crosses01:18

Dihybrid Crosses

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Related Experiment Video

Updated: Jul 9, 2026

Tomato Analyzer: A Useful Software Application to Collect Accurate and Detailed Morphological and Colorimetric Data from Two-dimensional Objects
15:25

Tomato Analyzer: A Useful Software Application to Collect Accurate and Detailed Morphological and Colorimetric Data from Two-dimensional Objects

Published on: March 16, 2010

Diversity in conserved genes in tomato.

Allen Van Deynze1, Kevin Stoffel, C Robin Buell

  • 1Seed Biotechnology Center, University of California, 1 Shields Ave,, Davis, CA, USA. avandeynze@ucdavis.edu

BMC Genomics
|December 20, 2007
PubMed
Summary

Researchers identified nucleotide variations in tomato breeding germplasm using conserved single-copy ESTs. This study provides valuable genetic markers for improving tomato traits through intra-specific breeding programs.

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Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
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Co-localization of Cell Lineage Markers and the Tomato Signal
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Co-localization of Cell Lineage Markers and the Tomato Signal

Published on: December 28, 2016

Related Experiment Videos

Last Updated: Jul 9, 2026

Tomato Analyzer: A Useful Software Application to Collect Accurate and Detailed Morphological and Colorimetric Data from Two-dimensional Objects
15:25

Tomato Analyzer: A Useful Software Application to Collect Accurate and Detailed Morphological and Colorimetric Data from Two-dimensional Objects

Published on: March 16, 2010

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
09:05

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Published on: March 11, 2020

Co-localization of Cell Lineage Markers and the Tomato Signal
10:56

Co-localization of Cell Lineage Markers and the Tomato Signal

Published on: December 28, 2016

Area of Science:

  • Plant genetics and genomics
  • Crop improvement
  • Molecular biology

Background:

  • Tomato possesses extensive genetic and genomic resources, including EST data and genetic maps.
  • Resource development has primarily focused on interspecific populations, leaving a gap in understanding intra-specific genotypic variation for breeding.

Purpose of the Study:

  • To identify nucleotide variation within tomato breeding germplasm and mapping parents.
  • To analyze conserved single-copy ESTs orthologous between tomato and Arabidopsis.

Main Methods:

  • Pooled sequencing strategy to screen 967 tomato transcripts.
  • Polymorphism screening in 12 diverse tomato lines.

Main Results:

  • Identified 1,487 single nucleotide polymorphisms and 282 insertion/deletions.
  • 579 SNPs and 206 indels were polymorphic in breeding germplasm.
  • Divergence observed between fresh market and processing germplasm, and between S. lycopersicum var. cerasiformae and S. pimpinellifolium.

Conclusions:

  • Significant progress made in bridging the gap between genetic resources for wide crosses and intra-specific breeding.
  • Identified polymorphisms serve as valuable marker resources for tomato breeding.
  • The approach is applicable to other Solanaceae crops.