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Related Concept Videos

Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...
Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Sequences01:29

Sequences

Sequences are fundamental mathematical objects consisting of ordered lists of numbers that follow a specific rule or pattern. Sequences are critical in various mathematical concepts, including calculus, series, and number theory. They can model real-world phenomena such as population growth, financial investments, and physical processes like the diminishing height of a bouncing ball.Each number in a sequence is referred to as a term. Typically, the terms are denoted as a1, a2, a3,…, where the...

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Related Experiment Video

Updated: Jul 9, 2026

Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web
09:51

Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web

Published on: July 16, 2017

The Personal Sequence Database: a suite of tools to create and maintain web-accessible sequence databases.

Scott A Givan1, Christopher M Sullivan, James C Carrington

  • 1Center for Genome Research and Biocomputing, Oregon State University, Corvallis, Oregon, USA. givans@cgrb.oregonstate.edu

BMC Bioinformatics
|December 20, 2007
PubMed
Summary

The Personal Sequence Database (PSD) allows scientists to easily create and manage private or public web-accessible molecular sequence databases. It simplifies data sharing and tracks new sequence similarities in public databases.

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Last Updated: Jul 9, 2026

Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web
09:51

Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web

Published on: July 16, 2017

An Integrated Approach for Microprotein Identification and Sequence Analysis
09:37

An Integrated Approach for Microprotein Identification and Sequence Analysis

Published on: July 12, 2022

The ITS2 Database
16:17

The ITS2 Database

Published on: March 12, 2012

Area of Science:

  • Bioinformatics
  • Molecular Biology
  • Genomics

Background:

  • Large molecular sequence databases are essential for bioscience research.
  • Maintaining smaller, project-specific sequence databases is beneficial but challenging for bench scientists.

Purpose of the Study:

  • To introduce the Personal Sequence Database (PSD) tool suite.
  • To enable scientists to create and manage personal web-accessible sequence databases.

Main Methods:

  • The PSD suite provides web-based tools for database creation and management.
  • Users can define private or public sequence groups.
  • Sequence groups support downloading, browsing, keyword searching, and BLAST similarity searches.

Main Results:

  • The PSD facilitates the creation and maintenance of small- to medium-sized sequence databases.
  • Users can manage private data or publish sequence groups for collaborators.
  • The BLASTAgent feature monitors public databases for sequence similarity updates.

Conclusions:

  • The PSD offers unique resources for managing sequence data and BLAST results.
  • It enhances data sharing among colleagues, collaborators, and the public.
  • The PSD is publicly available and hosted by the authors.