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Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)
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SNPs in PPARG associate with type 2 diabetes and interact with physical activity
Tuomas O Kilpeläinen1, Timo A Lakka, David E Laaksonen
1Institute of Biomedicine, Physiology, University of Kuopio, Kuopio, Finland. tuomas.kilpelainen@uku.fi
Medicine and Science in Sports and Exercise
|December 20, 2007
Summary
Certain single-nucleotide polymorphisms (SNPs) in the PPARG gene are linked to type 2 diabetes (T2D) risk. Increased physical activity (PA) may mitigate this risk, particularly for specific PPARG gene variants.
Area of Science:
- Genetics and Genomics
- Metabolic Diseases
- Preventive Medicine
Background:
- Type 2 diabetes (T2D) is a major global health concern.
- Genetic predisposition plays a role in T2D development.
- The peroxisome proliferator-activated receptor gamma (PPARG) gene is implicated in glucose metabolism.
Purpose of the Study:
- To investigate the association between specific single-nucleotide polymorphisms (SNPs) in the PPARG gene and the progression from impaired glucose tolerance (IGT) to T2D.
- To examine the potential interaction between these PPARG gene SNPs and physical activity (PA) in modulating T2D risk.
Main Methods:
- The study analyzed 479 overweight individuals with IGT from the Finnish Diabetes Prevention Study (DPS).
- Participants were followed for an average of 4.2 years.
- Yearly assessments of physical activity (PA) were conducted using a validated questionnaire.
Main Results:
- Two SNPs, rs17036314 and rs1801282 (Pro12Ala), were associated with an increased risk of conversion to T2D.
- The association for rs17036314 remained significant after adjusting for baseline fasting glucose.
- Increased physical activity significantly modified the risk associated with rs17036314 and rs1801282, seemingly negating the risk conferred by the associated alleles.
- A distinct SNP, rs1152003, showed an interaction with the study group, tending to increase T2D risk in the intervention group, but this was not modified by PA changes.
Conclusions:
- Specific PPARG gene SNPs (rs17036314, rs1801282, and rs1152003) are associated with T2D risk in the DPS cohort.
- Higher levels of physical activity appear to counteract the T2D risk associated with rs17036314 and rs1801282.
- The influence of rs1152003 on T2D risk may be modulated by broader lifestyle interventions or other lifestyle factors.
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