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Nonrandom chromosome changes in methylnitrosourea (MNU) induced mouse T-cell lymphomas
M Kaelbling1, Y D Zhang, U B Dasgupta
1Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, New York 10461.
Annales De Genetique
|January 1, 1991
Summary
Researchers studied chromosome changes in mouse T-cell lymphomas induced by methylnitrosourea (MNU). Specific chromosomes (X, 15, 14, 3, 12) showed consistent alterations, indicating their crucial role in cancer development.
Area of Science:
- Oncology
- Genetics
- Cancer Research
Background:
- Nonrandom chromosome alterations in neoplastic cells are key indicators of gene changes related to cancer transformation.
- Methylnitrosourea (MNU) is a chemical mutagen known to induce various cancers, including T-cell lymphomas.
Purpose of the Study:
- To investigate chromosomal abnormalities in T-cell lymphomas induced by MNU in RF/J mice.
- To identify specific chromosomes consistently altered during lymphoma development and progression.
Main Methods:
- Induction of thymic lymphomas in mice using MNU.
- Karyotypic analysis of thymus cells at various stages of lymphoma development.
- Chromosomal examination of tumor cells passaged in syngeneic and nude mice.
Main Results:
- Nonrandom numerical and structural chromosomal alterations were observed in direct thymus cell preparations.
- Specific chromosomes (X, 3, 15, 4, 8, 12, 14, 17) were frequently involved in initial lymphoma development.
- Chromosomes X, 15, 14, 3, and 12 consistently showed alterations in both direct and passaged cells, suggesting their critical role.
Conclusions:
- The X, 15, 14, 3, and 12 chromosomes are likely to harbor genes critical for neoplastic transformation in T-cell lymphomas.
- These findings highlight conserved chromosomal regions involved in cancer across species, such as the mouse chromosome 15 region containing Myc and Pvt-1 genes.