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Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT
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Published on: September 2, 2015

PTD-mediated delivery of anti-cell death proteins/peptides and therapeutic enzymes

Sadamitsu Asoh1, Shigeo Ohta

  • 1Department of Biochemistry and Cell Biology, Institute of Development and Aging Sciences, Graduate School of Medicine, Nippon Medical School, 1-396, Kosugi-cho, Nakahara-ku, Kawasaki-city, Kanagawa 211-8533, Japan. sada@nms.ac.jp

Advanced Drug Delivery Reviews
|December 21, 2007
PubMed

Insights

Apoptosis, programmed cell death, is crucial for survival, but its dysregulation causes disease. Protein transduction technology enables delivery of therapeutic proteins to treat these apoptosis-related conditions.

Area of Science:

  • Cellular biology
  • Molecular medicine
  • Biotechnology

Background:

  • Apoptosis (programmed cell death) is a vital physiological process essential for organismal survival.
  • Dysregulation of apoptosis is implicated in various diseases, including degenerative disorders, ischemic injuries, immunodeficiency, and infertility.
  • Understanding apoptosis signaling pathways provides targets for therapeutic intervention.

Purpose of the Study:

  • To explore the therapeutic potential of protein transduction technology for diseases linked to apoptosis.
  • To highlight the in vivo delivery of various therapeutic proteins using this technology.

Main Methods:

  • Utilizing protein transduction technology for the delivery of full-length proteins and peptides.
  • Administering therapeutic agents such as anti-apoptotic proteins, enzymes, and inhibitors in vivo.

Main Results:

  • Protein transduction technology facilitates the delivery of therapeutic proteins to diverse tissues, including the brain.
  • In vivo delivery of various therapeutic proteins and peptides has shown promise in mitigating disease models.
  • Specific examples include delivery of anti-apoptotic proteins, antioxidant enzymes, neuroprotectants, metabolic enzymes, and inhibitors of key signaling pathways.

Conclusions:

  • Protein transduction technology offers a viable strategy for delivering therapeutic proteins to combat diseases associated with apoptosis dysregulation.
  • This technology holds significant potential for developing novel treatments for a range of degenerative and injury-related conditions.

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