Related Experiment Video
Updated: Jul 9, 2026

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
Published on: October 15, 2015
Enhanced sulfate reduction with acidogenic sulfate-reducing bacteria
Aijie Wang1, Nanqi Ren, Xu Wang
1School of Municipal & Environmental Engineering, Harbin Institute of Technology, P.O. Box 2614, 202 Haihe Road, Harbin 150090, China.
Optimizing the chemical oxygen demand to sulfate ratio is key for effective sulfate reduction in wastewater. A critical ratio of 2.7 supports high sulfate removal rates by sulfate-reducing bacteria.
Area of Science:
- Environmental microbiology
- Biotechnology
- Wastewater treatment
Background:
- Sulfate reduction is crucial for treating sulfate-laden industrial wastewater.
- Molasses wastewater offers a viable carbon source for microbial sulfate removal.
- Optimizing reactor conditions is essential for efficient sulfate reduction.
Purpose of the Study:
- To investigate sulfate reduction efficiency in an acidogenic reactor using molasses wastewater.
- To determine the critical chemical oxygen demand to sulfate (COD/SO4(2-)) ratios for optimal performance.
- To understand the microbial metabolism involved in COD and sulfate degradation.
Main Methods:
- Continuous flow acidogenic reactor operation.
- Varying chemical oxygen demand/sulfate (COD/SO4(2-)) ratios.
- Analysis of microbial metabolism, including hydrogen and volatile fatty acid consumption.
Main Results:
- A critical COD/SO4(2-) ratio of 2.7 was identified for efficient sulfate removal (>94.6%).
- At this ratio, neither COD nor sulfate were in excess, indicating balanced microbial activity.
- An acetic-type microbial metabolism was established, with sulfate-reducing bacteria (SRB) consuming key intermediates.
- A lower critical ratio of 1.6 was observed with an enriched anaerobic SRB (ASRB) population.
Conclusions:
- The critical COD/SO4(2-) ratio significantly impacts sulfate reduction efficiency.
- Balanced substrate supply (COD and sulfate) promotes effective microbial sulfate removal.
- Enriched ASRB populations can enhance sulfate treatment capabilities even with limited COD.
More Related Videos
Related Concept Videos
Microbes and the Sulfur Cycle
Acid Mine Drainage
Sulfur Assimilation
Anoxygenic Photosynthesis
Microbes and Other Elemental Cycles
Anoxygenic Phototrophic Bacteria

