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Updated: Jul 9, 2026

Transgene Expression in Cultured Cells Using Unpurified Recombinant Adeno-Associated Viral Vectors
Published on: October 20, 2023
Relative influence of the adeno-associated virus (AAV) type 2 p5 element for recombinant AAV vector site-specific
Mickaël Guilbaud1, Gilliane Chadeuf, Fabio Avolio
1INSERM U758, Ecole Normale Supérieure de Lyon, 46 Allée d'Italie, F-69007 Lyon, France.
Abstract:
The p5 promoter region of the adeno-associated virus type 2 (AAV-2) rep gene has been described as essential for Rep-mediated site-specific integration (RMSSI) of plasmid sequences in human chromosome 19. We report here that insertion of a full-length or minimal p5 element between the viral inverted terminal repeats does not significantly increase RMSSI of a recombinant AAV (rAAV) vector after infection of growth-arrested or proliferating human cells. This result suggests that the p5 element may not improve RMSSI of rAAV vectors in vivo.

