Breast-cancer stromal cells with TP53 mutations and nodal metastases
- 1Genomic Medicine Institute, Cleveland Clinic Foundation, Cleveland 44195, USA.
- 0Genomic Medicine Institute, Cleveland Clinic Foundation, Cleveland 44195, USA.
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View abstract on PubMed
Summary
This summary is machine-generated.Genomic alterations in tumor stroma, specifically TP53 mutations and loss of heterozygosity, are linked to regional lymph-node metastases in sporadic breast cancer, but not hereditary breast cancer.
Area Of Science
- Oncology
- Genetics
- Cancer Microenvironment Research
Background
- The tumor microenvironment's role in cancer progression is increasingly recognized.
- The study investigates the impact of TP53 gene mutations and genomic alterations in stromal cells on breast cancer outcomes.
Purpose Of The Study
- To determine if TP53 gene inactivation and stromal genomic alterations correlate with clinical outcomes in breast cancer.
- To analyze compartment-specific genetic changes in both hereditary and sporadic breast cancer.
Main Methods
- TP53 mutation analysis and genomewide loss of heterozygosity/allelic imbalance analysis were performed on isolated epithelial and stromal cells.
- Data were collected from 43 hereditary and 175 sporadic breast cancer patients.
- Associations between genetic status, TP53 mutations, and clinical/pathological characteristics were computed.
Main Results
- TP53 mutations correlated with increased genomic instability in both hereditary and sporadic breast cancers.
- Stromal TP53 mutations were associated with regional nodal metastases in sporadic breast cancer.
- Specific genomic alterations in sporadic tumor stroma, independent of TP53 mutations, also predicted nodal metastases.
Conclusions
- Stroma-specific genomic instability, particularly loss of heterozygosity or allelic imbalance, is linked to TP53 mutations and lymph-node metastases in sporadic breast cancer.
- These associations were not observed in hereditary breast cancer.
- The findings highlight the distinct roles of stromal genetics in different breast cancer subtypes.
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