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Cells undergoing apoptosis form apoptotic bodies that must be removed immediately to prevent inflammation, autoimmune diseases, and necrosis. Phagocytosis is carried out by professional phagocytes such as macrophages or immature dendritic cells. Non-professional phagocytes such as epithelial cells and fibroblasts also take part in this process; however, they are not as effective as professional phagocytes.
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Related Experiment Video

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Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries

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Decrease in CD93 (C1qRp) expression in a human monocyte-like cell line (U937) treated with various apoptosis-inducing

Nobunao Ikewaki¹, Hidekazu Tamauchi, Hidetoshi Inoko

¹Kyushu University of Health, Welfare School of Health Science, Takahashi Educational Institute, Nobeoka, Miyazaki, Japan. immuneni@phoenix.ac.jp

Microbiology and Immunology

|December 21, 2007

Summary

Human CD93 expression significantly decreases on apoptotic myeloid cells, offering a new model to study immune cell regulation during apoptosis. This research explores CD93 modulation in U937 cells undergoing apoptosis induced by chemical agents.

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Published on: August 2, 2021

Area of Science:

Immunology
Cell Biology
Molecular Biology

Background:

Human CD93 (C1qRp) is a myeloid lineage receptor involved in phagocytosis and immune responses.
Previous studies showed protein kinase C (PKC) activators up-regulate CD93 expression.
The regulation of CD93 in apoptotic myeloid cells remains poorly understood.

Purpose of the Study:

To investigate the modulation of CD93 expression on human monocyte-like U937 cells during chemically induced apoptosis.
To analyze the secretion of soluble CD93 (sCD93) in apoptotic U937 cells.

Main Methods:

U937 cells were treated with apoptosis-inducing agents (ActD, CPT, CHX, EPS, MMC).
Apoptosis was assessed using Annexin V and 7-amino actinomycin D (7AAD) via flow cytometry.
CD93 expression was analyzed using flow cytometry and immunoblotting; sCD93 secretion was measured by EIA.

Main Results:

Apoptosis-inducing agents significantly decreased CD93 expression on U937 cells in early apoptosis.
Expressions of HLA-class I, HLA-class II, CD18, and CD54 were also decreased on apoptotic U937 cells.
Apoptotic U937 cells rapidly secreted sCD93 in the presence of PMA, but not otherwise.

Conclusions:

CD93 expression is dramatically decreased on U937 cells with apoptotic properties.
The decrease in CD93 expression on apoptotic myeloid cells provides a model for studying CD93 regulation.
This finding contributes to understanding immune responses during apoptosis.