Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Aryl hydrocarbon Receptor Nuclear Translocator 2: A Forgotten Per-ARNT-Sim Transcription Factor.

Toxicological sciences : an official journal of the Society of Toxicology·2026

Same author

Diabetes is associated with increased nocturnal respiratory rate.

medRxiv : the preprint server for health sciences·2026

Same author

Nocturnal Respiratory Rate and Variability Predict Long-term Mortality in Stable Outpatients with Cardiovascular Disease.

medRxiv : the preprint server for health sciences·2026

Same author

Spatial and Single-Cell Mapping Reveals Valvular Interstitial Cell and Macrophage Sex Differences in Calcific Aortic Valve Disease.

Arteriosclerosis, thrombosis, and vascular biology·2026

Same author

A triple-node heart-brain neuroimmune loop underlying myocardial infarction.

Cell·2026

Same author

Pesco-Vegetarian Food Components Promote Colonocyte Ferroptosis in Preclinical Mouse Models and a Randomized Crossover Trial in Healthy Human Adults.

The Journal of nutrition·2025

Search research articles

Related Experiment Video

Updated: Jul 9, 2026

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Published on: October 1, 2007

Microfluidic flow-encoded switching for parallel control of dynamic cellular microenvironments.

Kevin R King¹, Sihong Wang, Arul Jayaraman

¹Harvard-MIT, Division of Health Science and Technology, 51 Blosson St., Rm 408, Boston, MA 02114, USA.

|December 21, 2007

Summary

This summary is machine-generated.

Understanding how stimulus timing affects cellular responses is key. This study introduces a microfluidic system using Flow-encoded Switching (FES) to precisely control dynamic stimuli, revealing timing-dependent cell fate decisions.

More Related Videos

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Published on: April 17, 2021

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

Published on: October 6, 2019

Related Experiment Videos

Last Updated: Jul 9, 2026

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Published on: October 1, 2007

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Published on: April 17, 2021

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

Published on: October 6, 2019

Area of Science:

Cell Biology
Microfluidics
Systems Biology

Background:

The temporal dynamics of biological stimuli significantly influence cellular responses.
Precisely controlling dynamic cellular microenvironments is crucial for understanding cell behavior.

Purpose of the Study:

To develop a microfluidic system capable of delivering precisely controlled, dynamic temporal stimuli to cells.
To investigate the impact of stimulus timing on cellular responses, specifically NF-kappaB activation and cell fate.

Main Methods:

Development of a microfluidic parallel perfusion culture system with a "Flow-encoded Switching" (FES) design.
Utilizing laminar flow and diffusion-limited mixing to encode stimulus profiles via flow rate ratios controlled by differential pressure.
Live-cell imaging to monitor cellular responses to dynamic stimuli.

Main Results:

The FES system successfully delivered diverse temporal stimulus profiles to adherent cells.
NF-kappaB transcriptional activation and cell fate decisions were shown to be quantitatively and qualitatively dependent on stimulus timing.
Demonstrated the system's utility in probing the functional significance of temporally patterned cellular environments.

Conclusions:

Microfluidic Flow-encoded Switching (FES) provides a scalable method for creating dynamic cellular microenvironments.
The timing of stimuli plays a critical role in determining cellular transcriptional responses and fate decisions.
This technology enables systematic investigation into the functional importance of temporal patterns in cellular signaling.