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Updated: Jul 9, 2026

Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins
Published on: July 1, 2011
Azospirillum brasilense Sp7 produces an outer-membrane lectin that specifically binds to surface-exposed
Paola Mora1, Federico Rosconi, Laura Franco Fraguas
1Cátedra de Bioquímica, Departamento de Biociencias, Facultad de Química, CC 1157, Montevideo, Uruguay.
Abstract:
Azospirillum sp promotes the growth of many important crop plants. We demonstrated lectin binding activity in outer-membrane protein extracts of A. brasilense Sp7 by hemagglutination assays. The lectin specifically recognised the exopolysaccharide (EPS) produced by aggregated cells. Affinity chromatography using EPS-Sepharose was used to identify a 67 kDa outer-membrane lectin (OML) that recognised a binding region in the extracellular polysaccharide. Results show the specific recognition and binding between EPS and OML. The potential relationship between cell-to-cell aggregation and the OML-EPS interaction is discussed.
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