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[Kinetics study on intestinal absorption of bergenin in rats]
Xuan Qin1, Dan Zhou, Yuan Huang
1Key Laboratory of Drug Targeting of Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Objective:
To study the kinetics of intestine absorption of bergenin in rats.
Methods:
The intestine absorption of bergenin in the rats was determined by in situ perfusion. The HPLC were used to determine the concentration of bergenin in the perfusate and the plasma. The concentrations of bergenin in various sites of the intestine and the pH values were studied.
Results:
In the range of 0. 07-0. 21 mg/mL, the absorption of bergenin exhibited linear kinetics. The absorption varied in the duodenum, jejunum, ileum and colon. In the range of pH 5.40-7.80, with the increasing of pH value, the Ka of bergenin decreased.
Conclusion:
Bergenin is absorbed by the entire intestine, but with limited amount. The absorption of the drug is a first-order process with the passive diffusion mechanism.
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