[Detecting perfluorooctanoic acid by reversed phase high performance liquid chromatography with pre-column
Xiao-yan Cheng1, Ke-rong Zhang, Bo Zheng
1Department of Sanitary Technology, West China School of Public Health, Sichuan University, Changdu, China.
Objectives:
To establish a reversed phase high performance liquid chromatographic method with pre-column ultraviolet derivatization for the determination of perfluorooctanoic acids (PFOA) in sewage and urine.
Methods:
PFOA in sewage and urine formed an ion-pair with tetrabutylammonium (TBA) and were extracted into MTBE. The PFOA then reacted with omega-bromoacetophenone (omega-BAP) in acetonitrile and produced materials quantifiable by HPLC with UV detector.
Results:
The detection limit of the proposed method was 7.0 microg/L with a relative standard deviation of less than 10%. The recoveries for real samples ranged from 85.3% to 116%.
Conclusion:
The proposed method is suitable for the determination of PFOA in sewage and urine.
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