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Profiling the Monascus pilosus proteome during nitrogen limitation.

Wun-Yuan Lin1, Jui-Yun Chang, Chih-Hsuan Hish

  • 1Department of Food Science, Nutrition, and Nutraceutical Biotechnology, Shih Chien University, Taipei, Taiwan.

Journal of Agricultural and Food Chemistry
|December 22, 2007
PubMed
Summary
This summary is machine-generated.

Nitrogen limitation significantly suppresses red pigment production in Monascus pilosus, impacting metabolic pathways. This study reveals how Monascus adapts its metabolism under nutrient stress.

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Area of Science:

  • Microbial biotechnology
  • Metabolic engineering
  • Biochemistry

Background:

  • Monascus species are known for producing valuable secondary metabolites.
  • The effect of nitrogen limitation on Monascus secondary metabolite production is not well understood.

Purpose of the Study:

  • To investigate the impact of varying carbon/nitrogen (C/N) ratios on Monascus pilosus secondary metabolite production.
  • To elucidate the metabolic and proteomic changes in Monascus under nitrogen limitation.

Main Methods:

  • Cultivation of Monascus pilosus at different C/N ratios (20-60).
  • Comparative proteomic analysis using 2D gel electrophoresis and mass spectrometry (MALDI-TOF/TOF LC-MS/MS).

Main Results:

  • Red pigment production was significantly suppressed at a C/N ratio of 60, showing higher sensitivity than biomass growth.
  • Nitrogen limitation altered the expression of proteins involved in amino acid biosynthesis, protein translation, antioxidant defense, glycolysis, and transcriptional regulation.
  • Metabolic flux shifted from glycolysis to the tricarboxylic acid (TCA) cycle under nitrogen limitation.

Conclusions:

  • Nitrogen limitation triggers a metabolic shift in Monascus, repressing red pigment biosynthesis.
  • The study provides insights into the metabolic regulation of secondary metabolite production under nutrient stress.