Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Behavioral Tasks for Examining Identity Recognition In Mice06:58

Behavioral Tasks for Examining Identity Recognition In Mice

1.2K
By pairing negatively or positively valenced experiences with individual social targets, we developed behavior tasks for examining the identity recognition of C57BL/6 mice. These tasks allow for the study of mechanisms of social memory of individual social targets in healthy and disease-related mouse models with impaired social...
1.2K
Multiple Object Tracking05:55

Multiple Object Tracking

8.2K
Source: Laboratory of Jonathan Flombaum—Johns Hopkins University
8.2K
Combining Eye-tracking Data with an Analysis of Video Content from Free-viewing a Video of a Walk in an Urban Park Environment08:25

Combining Eye-tracking Data with an Analysis of Video Content from Free-viewing a Video of a Walk in an Urban Park Environment

9.6K
The objective of the protocol is to detail how to collect video data for use in the laboratory; how to record eye-tracking data of participants looking at the data and how to efficiently analyze the content of the videos that they were looking at using a machine learning...
9.6K
Novel Object Recognition and Object Location Behavioral Testing in Mice on a Budget05:57

Novel Object Recognition and Object Location Behavioral Testing in Mice on a Budget

58.8K
Here we provide a protocol which includes comprehensive instructions for the economical establishment of murine object location and novel object recognition behavioral testing, including the design, cost, and construction of required equipment as well as execution of behavioral testing, data collection, and...
58.8K
Novel Object Recognition Test for the Investigation of Learning and Memory in Mice08:52

Novel Object Recognition Test for the Investigation of Learning and Memory in Mice

77.1K
The object recognition test (ORT) is a simple and efficient assay for evaluating learning and memory in mice. The methodology is described...
77.1K
Creating Objects and Object Categories for Studying Perception and Perceptual Learning14:38

Creating Objects and Object Categories for Studying Perception and Perceptual Learning

12.2K
We describe a novel methodology for creating naturalistic 3-D objects and object categories with precisely defined feature variations. We use simulations of the biological processes of morphogenesis and phylogenesis to create novel, naturalistic virtual 3-D objects and object categories that can then be rendered as visual images or haptic...
12.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mild Neonatal Hypoxia Targets Synaptic Maturation, Disrupts Adult Hippocampal Learning and Memory, and Is Associated with CK2-Mediated Loss of Synaptic Calcium-Activated Potassium Channel KCNN2 Activity.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026
Same author

Diet, gut microbiome, and cognition in neurodegeneration: a review and methodological framework.

Frontiers in aging neuroscience·2026
Same author

Diet-Microbiome Relationships in Prostate-Cancer Survivors with Prior Androgen Deprivation-Therapy Exposure and Previous Exercise Intervention Enrollment.

Microorganisms·2026
Same author

Epigenetic and transcriptomic alterations precede amyloidosis in the Alzheimer's disease App<sup>NL-G-F</sup> knock-in mouse model.

Scientific reports·2025
Same author

Exercise, APOE Genotype, and Testosterone Modulate Gut Microbiome-Cognition Associations in Prostate Cancer Survivors.

Genes·2025
Same author

The Christchurch point mutation in mouse APOE reduces Aβ-induced tau and α-synuclein pathologies.

bioRxiv : the preprint server for biology·2025
Same journal

Time as the language of Behavior: events, sequences, patterns and meanings.

Journal of neuroscience methods·2026
Same journal

Detection of cochlear microphonic for differential diagnosis between auditory neuropathy mice and noise-induced sensorineural hearing loss mice.

Journal of neuroscience methods·2026
Same journal

Assessment metrics for pain control in rats: A methodological commentary.

Journal of neuroscience methods·2026
Same journal

Infant EEG preprocessing pipelines: A capability framework and current gaps in practice.

Journal of neuroscience methods·2026
Same journal

Methods for measuring neural activity during voluntary wheel running.

Journal of neuroscience methods·2026
Same journal

Serotype-dependent differences in AAV cellular transduction rates in the hypothalamus of Arctic ground squirrels.

Journal of neuroscience methods·2026
See all related articles

Related Experiment Video

Updated: Jan 20, 2026

Multiple Object Tracking: Studying Sustained Visual Attention
05:55

Multiple Object Tracking: Studying Sustained Visual Attention

Published on: April 29, 2023

8.2K

Object recognition analysis in mice using nose-point digital video tracking.

T S Benice1, Jacob Raber

  • 1Department of Behavioral Neuroscience, Oregon Health & Science University, Portland, OR 97239, USA.

Journal of Neuroscience Methods
|December 22, 2007
PubMed
Summary
This summary is machine-generated.

New nose-point video tracking software (NPVT) reliably automates object recognition memory testing in rodents. This automated method offers a more consistent alternative to manual scoring, improving data accuracy for behavioral neuroscience research.

More Related Videos

Novel Object Recognition and Object Location Behavioral Testing in Mice on a Budget
05:57

Novel Object Recognition and Object Location Behavioral Testing in Mice on a Budget

Published on: November 20, 2018

58.8K
Novel Object Recognition Test for the Investigation of Learning and Memory in Mice
08:52

Novel Object Recognition Test for the Investigation of Learning and Memory in Mice

Published on: August 30, 2017

77.1K

Related Experiment Videos

Last Updated: Jan 20, 2026

Multiple Object Tracking: Studying Sustained Visual Attention
05:55

Multiple Object Tracking: Studying Sustained Visual Attention

Published on: April 29, 2023

8.2K
Novel Object Recognition and Object Location Behavioral Testing in Mice on a Budget
05:57

Novel Object Recognition and Object Location Behavioral Testing in Mice on a Budget

Published on: November 20, 2018

58.8K
Novel Object Recognition Test for the Investigation of Learning and Memory in Mice
08:52

Novel Object Recognition Test for the Investigation of Learning and Memory in Mice

Published on: August 30, 2017

77.1K

Area of Science:

  • Neuroscience
  • Behavioral Science
  • Computational Biology

Background:

  • Rodent object recognition memory is crucial for understanding cognitive function.
  • Manual scoring of object recognition tasks is labor-intensive and prone to variability.
  • Novelty-seeking behavior in rodents is a key indicator in memory tests.

Purpose of the Study:

  • To evaluate the efficacy of novel nose-point video tracking software (NPVT) for automated data collection in rodent object recognition memory tasks.
  • To compare the reliability and validity of NPVT data against human observers with varying expertise.
  • To assess NPVT's performance in a specific genetic model relevant to cognitive decline.

Main Methods:

  • Mice were subjected to a novel object/novel location (NO/NL) task.
  • Data on object exploration time was collected using NPVT and manually by two expert and one inexperienced observer.
  • Correlation analyses were performed to compare data from NPVT and human observers.
  • NPVT results were validated using a NO/NL experiment involving mice with different human apolipoprotein E (APOE) genotypes.

Main Results:

  • NPVT data showed strong correlations with expert observers, indicating high reliability.
  • An inexperienced observer's data exhibited poor correlation with both NPVT and expert observers.
  • NPVT and expert observers yielded similar group average results, unlike the inexperienced observer.
  • NPVT successfully differentiated performance in mice expressing APOE3 versus APOE4, a known risk factor for cognitive decline.

Conclusions:

  • Nose-point video tracking software (NPVT) provides a reliable and valid automated method for analyzing rodent object recognition memory.
  • NPVT reduces inter-observer variability and offers a more efficient alternative to manual scoring.
  • This technology has potential applications in various rodent behavioral tests, aiding cognitive research and drug discovery.