Related Experiment Video
Updated: Jul 9, 2026

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
Ethanol production from maize silage as lignocellulosic biomass in anaerobically digested and wet-oxidized manure
Piotr Oleskowicz-Popiel1, Przemyslaw Lisiecki, Jens Bo Holm-Nielsen
1Biosystems Department, Risoe National Laboratory, Technical University of Denmark, DTU, Frederiksborgvej 399, 4000 Roskilde, Denmark. piotr.o.popiel@risoe.dk
Abstract:
In this communication, pretreatment of the anaerobically digested (AD) manure and the application of the pretreated AD manure as liquid medium for the simultaneous saccharification and fermentation (SSF) were described. Furthermore, fermentation of pretreated maize silage and wheat straw was investigated using 2l bioreactors. Wet oxidation performed for 20 min at 121 degrees C was found as the most suitable pretreatment conditions for AD manure. High ammonia concentration and significant amount of macro- and micro-nutrients in the AD manure had a positive influence on the ethanol fermentation. No extra nitrogen source was needed in the fermentation broth. It was shown that the AD manure could successfully substitute process water in SSF of pretreated lignocellulosic fibres. Theoretical ethanol yields of 82% were achieved, giving 30.8 kg ethanol per 100 kg dry mass of maize silage.
Related Concept Videos
Production of Alcohol
Production of Organic Acids
Bioreactor Controls-III
Microbes and Methanogenesis
Microbes in the Production of Fermented Foods
Fates of Pyruvate
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...

