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Kinking the double helix by bending deformation.

Quan Du1, Alexander Kotlyar, Alexander Vologodskii

  • 1Department of Chemistry, New York University, New York, NY 10003, USA.

Nucleic Acids Research
|December 22, 2007
PubMed
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Strong DNA bending disrupts the regular helical structure, especially in smaller DNA circles. This study reveals that bending stress causes local disruptions when DNA curvature radius is less than 3.5 nm.

Area of Science:

  • Molecular Biology
  • Biophysics
  • Structural Biology

Background:

  • DNA structure is susceptible to deformations like bending and torsional stress during cellular functions.
  • While torsional stress effects are well-studied, the impact of bending stress on DNA structure remains largely theoretical.
  • Understanding these deformations is crucial for comprehending DNA mechanics and function.

Purpose of the Study:

  • To experimentally investigate the effects of bending stress on DNA helical structure.
  • To determine the critical curvature radius at which bending stress disrupts DNA.
  • To analyze the structural consequences of DNA bending in minicircles.

Main Methods:

  • Development of an efficient method for producing covalently closed DNA minicircles.

Related Experiment Videos

  • Utilizing single-strand-specific endonucleases to detect structural disruptions within DNA minicircles.
  • Comparing structural integrity in minicircles of different sizes (64-65 bp vs. 85-86 bp).

    • Main Results:

    • Bending stress was found to disrupt the regular DNA helical structure when the radius of curvature was less than 3.5 nm.
    • Structural disruptions were observed in 64-65 bp minicircles, indicating sensitivity to bending deformation.
    • Larger 85-86 bp minicircles did not show significant disruption under similar bending stress.
    • Base pairing was preserved despite the observed disruptions in helical structure.

    Conclusions:

    • Significant DNA bending induces local structural disruptions, characterized by kink formation.
    • The critical radius for DNA structural disruption by bending is approximately 3.5 nm.
    • DNA minicircle size influences its susceptibility to bending-induced structural changes, preserving base pairing.