Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
Lineage Commitment01:21

Lineage Commitment

Commitment is the  process whereby stem cells:
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
Primary Lymphoid Organs01:16

Primary Lymphoid Organs

Primary lymphoid organs are pivotal in the formation, development, and maturation of lymphocytes, the white blood cells that serve as the backbone of our immune system. This crucial function underscores their fundamental role in maintaining our overall health and immunity. The two primary lymphoid organs of prime importance are the red bone marrow and the thymus.
The red bone marrow is a soft, spongy tissue nestled in the interior of long bones such as the humerus and femur. It is the site...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Disrupting Treg lineage stability elicits RORgT-mediated plasticity and enhances anti-tumor immunity.

Cell death & disease·2026
Same author

An integrated hydrogel-V3 interneuron therapy promotes functional repair of spinal cord injury via neural circuit reconstruction and microenvironment remodeling.

Bioactive materials·2026
Same author

Comparative effectiveness of different inhaler technique education modalities on clinical outcomes in patients with asthma and chronic obstructive pulmonary disease: a protocol for a systematic review and network meta-analysis of randomised controlled trials.

BMJ open·2026
Same author

GOLDEN fusion: a graph-oriented learning with domain-embedding network fusion for generating super gene sets in functional genomics.

Briefings in bioinformatics·2026
Same author

Acetonitrile-driven interface force field reconstruction of HLB-SPME: insight into the pesticide adsorption mechanism based on energy lattice point models.

Analytical methods : advancing methods and applications·2026
Same author

An AI-Enabled Single-Cell Transcriptomic Analysis Pipeline for Gene Signature Discovery in Natural Killer Cells Linked to Remission Outcomes in Chronic Myeloid Leukemia.

Biology·2026

Related Experiment Video

Updated: Jul 9, 2026

Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production
08:22

Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production

Published on: May 31, 2020

Retinoids accelerate B lineage lymphoid differentiation.

Xinrong Chen1, Brandt L Esplin, Karla P Garrett

  • 1Immunobiology and Cancer Program, Oklahoma Medical Research Foundation, Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|December 22, 2007
PubMed
Summary
This summary is machine-generated.

All-trans retinoic acid (ATRA) significantly impacts B cell development. This study reveals ATRA accelerates differentiation of B lineage cells while inhibiting progenitor expansion, suggesting a role for retinoids in the immune system.

More Related Videos

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
07:12

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

Radial Mobility and Cytotoxic Function of Retroviral Replicating Vector Transduced, Non-adherent Alloresponsive T Lymphocytes
10:01

Radial Mobility and Cytotoxic Function of Retroviral Replicating Vector Transduced, Non-adherent Alloresponsive T Lymphocytes

Published on: February 11, 2015

Related Experiment Videos

Last Updated: Jul 9, 2026

Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production
08:22

Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production

Published on: May 31, 2020

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
07:12

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

Radial Mobility and Cytotoxic Function of Retroviral Replicating Vector Transduced, Non-adherent Alloresponsive T Lymphocytes
10:01

Radial Mobility and Cytotoxic Function of Retroviral Replicating Vector Transduced, Non-adherent Alloresponsive T Lymphocytes

Published on: February 11, 2015

Area of Science:

  • Immunology
  • Hematology
  • Molecular Biology

Background:

  • Retinoids are known to affect hemopoietic stem cell integrity.
  • The influence of retinoids on immune system progenitor cells requires further investigation.

Purpose of the Study:

  • To investigate the effects of all-trans retinoic acid (ATRA) on immune cell development, specifically B lineage cells.
  • To determine the role of retinoids in replenishing the immune system.

Main Methods:

  • Treatment of C57BL6 mice with ATRA.
  • In vitro culture of B lymphoid progenitors with ATRA.
  • Analysis of cell populations, proliferation, and differentiation markers (e.g., CD19+).
  • PCR analysis and treatment with retinoid acid receptor (RAR)/retinoid X receptor agonists.
  • Assessment of human B cell differentiation.

Main Results:

  • ATRA treatment led to a substantial increase in CD19+ B lineage cells in mouse marrow and spleens.
  • Lymphoid progenitors were reduced, and B lymphoid progenitors were identified as direct targets of ATRA.
  • ATRA shortened the differentiation time for primitive progenitors to generate CD19+ cells.
  • Retinoid acid receptor alpha (RARα) was implicated as the mediator of these responses.
  • Transcription factors EBF1 and Pax-5 were elevated following ATRA treatment.
  • Similar effects were observed in human B cell differentiation, with inhibited progenitor expansion and accelerated differentiation.

Conclusions:

  • All-trans retinoic acid (ATRA) influences B cell development by accelerating differentiation and inhibiting progenitor expansion.
  • Retinoid acid receptor alpha (RARα) plays a key role in mediating ATRA's effects on B cell lymphopoiesis.
  • These findings suggest retinoids contribute to the normal lymphopoietic environment in bone marrow and highlight potential side effects of ATRA therapy.