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Updated: Jul 9, 2026
In Ovo Intravascular Injection in Chicken Embryos
Published on: June 3, 2022
Effects of 17beta-estradiol on distribution of primordial germ cell migration in male chicks
Xiu-Mei Jin1, Yi-Xiang Zhang, Zan-Dong Li
1College of Biological Sciences, China Agricultural University, Yuan-Ming Yuan West Road 2, Beijing, China.
Aim:
To assess whether exogenous estradiol has any effect on migration of primordial germ cells (PGCs) in the chick.
Methods:
Fertilized eggs were treated with 17beta-estradiol (E(2)) (80 microg/egg) at stage X (day 0 of incubation), stages 8-10 (incubation 30 h) and 13-15 (incubation 55 h). Controls received vehicle (emulsion) only. Changes in PGC number were measured on different days according to developmental stages.
Results:
In male right gonads, but not in female left gonads, at stages 28-30 (incubation 132 h) significant decreases in the mean number of PGCs aggregating were observed compared with the controls (P < 0.05) while the total PGC number in the right and left gonads at each stage did not change (P > 0.05).
Conclusion:
The present study provides evidence that E(2) has significant effects on the localization of PGCs in male right, but not female left, gonads of chicken embryos at stages 28-30, compared with controls.
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