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Updated: Jul 9, 2026
Preparation of Mouse Retinal Cryo-sections for Immunohistochemistry
Published on: July 1, 2019
Development of an optimal antigen retrieval protocol for immunohistochemistry of retinoblastoma protein (pRB) in
1Department of Pathology, University of Southern California Keck School of Medicine, Los Angeles, California, USA.
Abstract:
A novel protocol for antigen retrieval (AR) for immunohistochemistry (IHC) of retinoblastoma protein (pRB) in formalin fixed, paraffin embedded (FFPE) tissue sections was developed using 0.05% citraconic anhydride as the AR solution for heat treatment based on comparison of different methods. This new protocol has advantages including superior morphological preservation, greater reproducibility, and more intense staining after retrieval. Our study demonstrates the importance of comparing various AR protocols to obtain maximal IHC for standardization and for quantitative IHC.
