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Lack of evidence for a saturable tetracycline transport system in Staphylococcus aureus
1Department of Microbial Biochemistry and Genetics, Lederle Laboratories, American Cyanamid, Pearl River, New York 10965.
Antimicrobial Agents and Chemotherapy
|December 1, 1991
Abstract:
Previous studies on tetracycline transport into Staphylococcus aureus identified a high-affinity, saturable uptake system for the antibiotic (Km, 4.76 microM) (B.L. Hutchings, Biochim. Biophys. Acta 174:734-738, 1969). However, the earlier results could not be confirmed using conditions that permitted energy-dependent, concentrative uptake of tetracycline. Kinetic artifacts introduced by inappropriate washing procedures may explain the previous results.