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Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
Published on: July 31, 2011
Detection and assay of some human proteins by immunoconcentration technique
1Biochemical Laboratory, Polish Academy of Sciences, Wrocław.
Abstract:
For detection and semi-quantitative assays of some human proteins by immunoconcentration techniques, specific antibodies covalently bound to nylon microparticles and then captured in the form of small dots on glass microfibre disc, were used. The discs were placed on a water absorbant material in a simple plastic device. For the technique, antibodies labeled with peroxidase were also applied. The use of several chromogenic substrates for peroxidase was checked in the technique.
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