Calpain II in rat peripheral nerve

We used a polyclonal antiserum directed against calpain II to study the distribution of that enzyme in rat sciatic nerve. Western blot of nerve homogenate showed that the antibody reacted with a single protein band of 80 kDa, corresponding to the catalytic subunit of calpain II. By light microscopy, immunoreactivity appeared predominantly in Schwann cell cytoplasm. By electron microscopy, calpain II was especially dense along the plasmalemma of Schwann cells, and was also seen in axoplasm.