Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A computer program for microplate reader interface and analysis of kinetic, microclot lysis assays.

P R Swank1, M L Lewis, K L Damron

  • 1KRUG Life Sciences, Houston, Texas 77058.

Thrombosis Research
|October 15, 1991
PubMed
Summary

This study details software for automated microplate readers to measure clot lysis time using optical density. This program significantly reduces technician time compared to manual methods.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phylogenetics of Australasian gall flies (Diptera: Fergusoninidae): Evolutionary patterns of host-shifting and gall morphology.

Molecular phylogenetics and evolution·2017
Same author

Genetic differentiation associated with host plants and geography among six widespread species of South American Blepharoneura fruit flies (Tephritidae).

Journal of evolutionary biology·2017
Same author

The "Better" Technique for Accessory Trocar Insertion.

Journal of minimally invasive gynecology·2016
Same author

Bristol Stool Form Scale reliability and agreement decreases when determining Rome III stool form designations.

Neurogastroenterology and motility·2015
Same author

A comparison of two approaches to education about carrier testing for cystic fibrosis.

Journal of genetic counseling·2013
Same author

The interpretation of Rome III criteria and method of assessment affect the irritable bowel syndrome classification of children.

Alimentary pharmacology & therapeutics·2010

Area of Science:

  • Biotechnology
  • Medical Technology
  • Computer Science

Background:

  • Automated microplate readers are crucial for high-throughput biological assays.
  • Accurate measurement of clot lysis time is important in hemostasis research.
  • Manual data analysis from microplate readers is time-consuming and prone to error.

Purpose of the Study:

  • To develop and implement a computer program for data acquisition and analysis from an automated microplate reader.
  • To determine the clot half-lysis time using optical density readings from microplate wells.
  • To assess the efficiency gains of the automated system over non-automated methods.

Main Methods:

  • Development of a software program for the APPLE IIe computer.
  • Integration of the software with an automated microplate reader.

Related Experiment Videos

  • Measurement of clot lysis by optical density readings over time.
  • Calculation of clot half-lysis time using recorded data.

    • Main Results:

    • The developed software successfully records and analyzes data from the microplate reader.
    • The program accurately determines the time required for clots to reach half lysis.
    • Significant savings in technician time were achieved compared to non-automated systems.

    Conclusions:

    • The developed software provides an efficient and accurate method for determining clot lysis time.
    • Automation of data analysis from microplate readers can lead to substantial time savings in laboratory settings.
    • This approach enhances the throughput and reliability of hemostasis assays.