Related Experiment Videos
In vitro expression of belladonna mottle virus genome
1Department of Biochemistry, Indian Institute of Science, Bangalore.
Indian Journal of Biochemistry & Biophysics
|October 1, 1991
Abstract:
In vitro translation of belladonna mottle virus BDMV(I) genomic RNA in a rabbit reticulocyte lysate system produced proteins of Mr 210,000, 150,000 and 78,000 which form the non-structural proteins. The coat protein, on the other hand, was expressed from a subgenomic RNA which was found to be encapsidated in the empty capsids forming the top component viral particles. The implications of subgenomic RNA encapsidation in viral replication and assembly are discussed.