Root canal sealer cytotoxicity on human gingival fibroblasts: 2. Silicone- and resin-based sealers

The cytotoxicity of two resin (AH26 and Diaket)- and one silicone (Lee Endo-Fill)-based root canal sealers were tested over a period of 21 days. The incorporation rate of L-[14C]leucine was measured to establish the potential of human gingival fibroblasts to synthesize protein after being in contact with the sealers. The cells were gained from biopsies from healthy patients between 20 and 30 years of age. The sealers were allowed to set for 24 and 48 h before dispensing the fibroblastic suspension into the culture dishes. AH26 showed in the 24-h group a severe cytotoxic reaction during the entire experimental period. Diaket showed a relative severe cytotoxic potential with slight cell recovery after 3 days of culturing. Lee Endo-Fill showed a significantly lower (p less than 0.05) cytotoxic potential during the first 11 days of incubation. However, between the 15th and 21st days of culturing an ascending cytotoxic potential was registered. With Diaket and Lee Endo-Fill a relative lower cytotoxic response was observed in the 48-h group; however, cytotoxic patterns similar to the 24-h group prevailed.